Irena Vekstein, customer service manager at Wigan Wickes, said: "It'll be a tough challenge, but everyone at the store is really looking forward to it. It's a great way of getting everyone together and raising money for an incredibly worthwhile charity."
A team of staff from Warrington Wickes will go on to walk to Northwich, the next store on the route, on 4 June, with the relay ending on 26 June in Havant.
People can support the challenge by donating at www.justgiving.co.uk/Great-Wickes-Walk
Wickes announced Leukaemia and Lymphoma Research as its first-ever national charity partnership in October 2006, aiming to raise £200 000 for the blood cancer charity during a year-long partnership.